FAQ
1. What is Bisulfite Sequence Mapping?
Currently a huge amount of bisulfite treated reads have been generated in the attempt to understand which specific base pairs in a given genome are methylated. Bisulfite sequencer will take these millions of reads and map them onto a reference genome and will take into account conversions from cytosine to thymine as well as allowing for mismatches. Currently we allow for 2 mismatches.
2. Will Bisulfite Sequencer miss detection of some reads?
Currently yes, we will miss detection of certain types of input read sequences. Because of the algorithm we use, if one third of the sequence is of unusually low complexity after converting thymine to cytosine, i.e. a long string of c's, then these read sequences will be thrown out.