Cell fate inclination within 2-cell and 4-cell mouse embryos
revealed by single-cell RNA sequencing
Fernando H. Biase, Xiaoyi Cao, Sheng Zhong
University
of California San Diego, UCSD
Department of Bioengineering
Supplemental
files:
Expression of GADD45A in 4-cell mouse embryos
Expression of ACOX3 in mouse embryos
Embryo 1 .tiff
Embryo 2,3 .tiff
Embryo 4 .tiff
Expression of AP2S1 in mouse embryos
Embryo 1 .tiff
Embryo 2 .tiff
Embryo 3 .tiff
Expression of ZFP688 in mouse embryos
Embryo 1 .tiff
Embryo 2 .tiff
Embryo 3 .tiff
Negative control of the
immunocytochemistry (no primary antibody)
Blastomere 1 .tiff
Embryo 1, 2 .tiff
Expression data (FPKM) of single cell RNA-sequencing from 2- and
4-cell mouse embryos.
Two-cells (n=10), 8,208 genes with FPKM>1 in 1 blastomere
of all embryos: file
Four-cells (n=5), 8,052 genes with FPKM>1 in 2
blastomeres of all embryos: file
Unfiltered expression data (FPKM)
Zygotes: file
Two-cells: file
Four-cells: file
Genes associated to embryonic genome activation (EGA), and stage
specific mRNA depletion
Number of genes and criteria of selection:
Oocytes (n=5), 10,406 genes with FPKM>1 in 3 oocytes
Zygotes (n=9), 9,834 genes with FPKM>1 in 5 zygotes
Two-cells (n=10), 8,208 genes with FPKM>1 in 1 blastomere
of all embryos
Four-cells (n=5), 8,052 genes with FPKM>1 in 2
blastomeres of all embryos
File 1- Embryo genomic
activation: Genes with expression detected in 1-, 2, and 4-cell stage but not
in oocytes.
File 2- Depletion of
mRNA between 1- and 2- cell stages.
File 3- Depletion of
mRNA between 2- and 4- cell stages.